Ran Group 2 Library 1 on a Flongle flow cell to confirm successful library prep.
Performed hardware check on Flongle adapter. Then inserted flongle flow cell and performed flow cell check.
Flongle flow cell:
ID: AYW871
61 pores on first pore check
57 pores on this pore check
According to TapeStation peaks these samples are ~700bp in avg fragment length. Thus, the recommended Flongle loading amount of 5-10fmol is roughly equivalent to 2.16ng - 4.31ng.
I loaded the Flongle with 10fmol (4.31ng) of Group 2 Library 1 in 5uL of EB. G2L1 concentration is too high to accurately pipette 4.31ng, so instead mixed:
\[ \frac{4.3ng\ DNA}{5uL\ EB} = \frac{20ng\ DNA}{23.3uL\ EB}\ \ \ Added\ 1uL\ G2L1\ to\ (23.3-1)=22.3uL\ EB \]