Checked Group 2 Library 2 on a Flongle flow cell.
Flow cell:
ID: AYX067
79 pores on pore check
Based on TapeStation peaks, samples are ~700bp, so 10fmol = 4.31ng.
\[ \frac{4.31ng\ DNA}{5uL\ EB} = \frac{21.55ng\ DNA}{25uL\ EB} \]
Then calculate volume of G2L2 needed
\[ \frac{21.55ng}{24.2 \frac{ng}{uL}} = 0.89uL\ input \]
Can approximate requisite input to 1uL, so mixed 1uL G2L2 with 24uL EB (total 25uL).
Loaded Flongle with 5uL of this.