After using most of my 1800s DNA in a partially unsuccessful library prep (Group 3 Library 1), I want extra for additional preps. I need to extract more DNa for 5 specimens:
| Catalog # | Collection Year |
|---|---|
| 50603 | 1912 |
| 19054 | 1898 |
| 42137 | 1897 |
| 14399 | 1886 |
| 50368 | 1880 |
Subsampled and began tissue digestion, as detailed here.
Began digestion at 7:00pm, 09/01/2025, will run for ~48hr.
Notes:
A power outage interrupted digestion incubation. Incubator stopped at ~9pm 09/02/25, and I restarted it ~7am 09/03/25, so digestions were sitting at room temp for ~10hrs.
Spiked in 20uL proteinase K to each tube, will let digestions sit on shaking incubator for another 12-24hr.