Ran G4L1 on Flongle flow cell to confirm successful library prep. Following gentle, negative, pressure, loading protocol created by Nanopore community members.
I also want to try loading more DNA on the Flongle, as I’ve seen recommended in community forum posts (and in the gentle Flongle loading protocol), so instead of the recommended 10fmol DNA, I’ll prep 30fmol in 5ul EB.
G4L1 is 59.2ng/ul, with an average length of 609bp, so concentration is 157.8 fmol/ul
\[ \frac{30fmol}{5uL\ EB} = \frac{157.8fmol}{26.3L\ EB} \]
So mix 1ul G4L1 with (26.3-1)=25.3ul EB , then use 5ul for loading.
Flongle Flow cell
[ID] AXT657
87 pores on 09/01/25 check
62 pores after loading
Still running on the MK1B sequencer after error issues with the MKID (see post).