Flongle Run, Group 4 Library 2

Run Flongle flow cell with G4L2 to confirm library prep.

Again, will use 30 fmol input DNA and gentle negative pressure loading protocol (print-out in back of lab notebook)

G4L2 has avg sample length of 609bp, concentration of 22 ng/ml => 58.64 fmol/ul

\[ \frac{30fmol\ DNA}{5uL\ EB} \Rightarrow \frac{58.64fmol}{9.77uL} \]

So mix 1ul G4L2 with (9.77ul-1) = 8.77ul EB then use 5ul.

Flowcell
ID: AXT6SS
82 pores on 09/01/25 check
84 pores on 09/08/25 check
50 pores after loading

Again, back to running on MKID.

Notes:

• Good occupancy on start though ~80% after 45min.

• Already seeing accumulation of “unavailable” pores