31 March 2025
Monday
1 hr – SWED103 class
3 hrs – catch up on misc tasks from my week out. Registration details, SIFP details (formally accept, contact andrea, etc.), catch up on slack messages and emails, set up planner for the quarter, check course syllabi/expectations/due dates, etc.
4 hr – trialing Ariana’s machine learning approach w time series Apul phys data and gene/miRNA counts. See work here
28 March 2025
Friday
Out
27 March 2025
Thursday
Out
26 March 2025
Wednesday
Out
25 March 2025
Tuesday
Out
24 March 2025
Monday
Out
3 hrs – QSCI381 finished grading final exam (did this on Sunday, but counting it as part of this workweek)
Recieved official notice of recieving SIFP!!!! The first fellowship I’ve won in grad school!!!!! Will be coordinating with Andrea once I’m back from vacation to go over details
21 March 2025
Friday
- 10.5 hr – QSCI381 grading final exam
20 March 2025
Thursday
5 hr – EPIMAR, prep for lab meeting, trying out a bunch of different visualizations to see what makes sense (cytoscape network, bubble plots, etc.)
0.5 hr – QSCI381 grading meeting
2 hr – lab meeting, talking about timeseries data synthesis/focus for EPIMAR
Daily total: 7.5 hr
19 March 2025
Wednesday
- 12 hrs – E5 timeseries data – functional enrichment of modules, annotating putative miRNA interaction, etc.
Daily total: 12 hr
18 March 2025
Tuesday
2.5 hrs – Booking travel for EPIMAR 2025, paying for registration, compiling relevant documentation for conference attendance and acceptance, receipts, etc. and submitting materials to TREQ for reimbursement. Will not be booking lodging for now because may utilize the PI lodging once the PI meeting is over
0.5 hr – QSCI381 misc
6 hrs – E5 EPIMAR stuff. Doing more checks of module eigengene behavior across different environmental/physiological variables, finding and saving important dataframes from WGCNA analysis, generating list of testable hypotheses to focus on answering during EPIMAR presentation.
0.5 hr – weekly meeting w Steven
Daily total: 9.5 hr
17 March 2025
Monday
- 7 hrs – QSCI381 Grading Assignment 9, answering a bunch of messages from students about the friday due date, looking over final exam key to prep for grading
Daily total: 7 hrs
14 March 2025
Friday
2 hr – E5 deep-dive expression work, trying different approach for siRNA fasta
1 hr – E5 biweekly meeting
1.5 hr – SWED102 class
3 hr – E5, reading again how ShortStack considers siRNA/IDs siRNA clusters (still not entirely clear to me). Lit review on siRNA in general.
It’s not actually that late in the day, but I just realized I’m over my (self-imposed) 50 hr/wk limit, so ending for the day.
Daily total: 7.5 hr
13 March 2025
Thursday
1.5 hr – SWED102 final prep
1 hr – SWED 102 final exam
0.5 hr – QSCI381 weekly meeting
1 hr – QSCI381 handling late (+extension) assignment submissions and grading, some email questions, tallying extra credit Ed discussion responses
2 hr – Lab meeting + creatigng template materials for Roberts Lab website undergraduate guide
2 hr – E5 timeseries work
Daily total: 8 hr
12 March 2025
Wednesday
1.5 hr – SWED102 class
2 hr – SWED102, second version of skrivuppgift 2 writing assignment
3 hr – SWED102 catch up on last HW assignments
2 hr – QSCI381 office hours
3 hr – E5 timeseries, running module-module comparisons, see WGCNA summary here
Daily total: 11.5 h
11 March 2025
Tuesday
2 hr – QSCI381 grading Assignment 8
1.5 hr – SWED102 class
1 hr – QSCI 381 ed discussion, canvas inbox, regrade requests
1 hr – Adding timepoints to E5 timeseries WGCNA, looking into shared modules being correlated with temp and timepoint. No correlation w solar radiation of rainfall suggests temp should be driving differential gene expression by timepoint. DGE analysis showed TP2 and TP4 as having most DEGs, but TP2 and TP3 have the two temperature extremes (highest and lowest) and are the only TPs significantly correlated with modules of gene expression. Maybe this is because both hot and cold extremes are handled by the same pathways, so TP2 and 3 have very similar “dealing with temp stress” expression profiles that contrast with TP1 and 4 “we’re at a comfortable temp” expression profiles?
Also notable, while TP2 and TP3 are significantly correlated with some of the same modules, they have opposite expression profile (upregulation vs. downregulation! Does this suggest that, while both warm and cool temperature stress may be associated with similar pathways, the way those pathways are modulated in differs when in response to different types of temp stress (e.g. gene is upregulated in response to heat stress and downregulated in response to cold stress)?- Edit 3/18/2025 – realized after plotting out eigengenes of each module that this ^^ is a misinterpretation of the heatmap – shared modules between TP2 and TP3 actually have matching expression profiles. This suggests shared pathways for both warm and cool temperature response and that the pathways are modulated in similar ways in response to both forms of temperature change.
2 hr – QSCI381 lab
0.5 hr – weekly meeting w Steven
0.5 hr – Just announced that Trump admin gutted the Department of Education, leaving it at ~50% of original workforce, so I finally took a moment to check on my student loans and download digital copies of all relevant documentation, just in case.
2.5 hr – working on E5 timeseries WGCNA (fixing data prep, correlating WGCNA modules, etc)
Daily total: 11 hrs
10 March 2025
Monday
1 hr – SWED102 class
0.5 hr – go through weekend’s emails/canvas inbox messages/ed discussion posts
4 hrs – QSCI 381 lab prep (reviewing weekly material, creating lab 9 key, updating canvas lab pages that were out of date, creating exam prep material for review this week)
2.5 hr – QSCI381 lab
6 hr – QSCI381 grading assignment 8
1 hr – QSCI381 misc (checked Assignment 9 key, created Assignment 9 gradescope submission form and Canvas assignment, linked them, updated due dates bc last day of instruction falls on a friday, sent some announcements about assignment 9 and my lab sections)
Daily total: 15 hours
07 March 2025
Friday
1 hr – SWED102 class
3 hr – SWED102 writing assignment 2 and quiz
4 hr – Stand up for Science rally at Seattle Center
Daily total: 8 hr
06 March 2025
Thursday
1 hr – SWED102 class
1.5 hr – FISH513 lit review/compilations
0.5 hr – QSCI381 weekly meeting (just Tsering today)
1 hr – Lab meeting
2 hr – FISH513 class
1 hr – SWED102 skrivuppgift 2 work
1 hr – attended department Q&A for the current knowledge/status of school, college, and university in the context of the EOs and federal ganecy changes
Daily Total: 8 hr
Went home a bit early because the Q&A meeting stressed me tf out (even though it was largely neutral) and I need to take a long walk.
05 March 2025
Wednesday
1 hr – SWED102
2 hr – E5 timeseries, rerunning some WGCNAs with environmental variables incorporated, plotting eigengene expressions over time, tryign to debug Cytoscape export – might just switch to different interaction network tool that I can use in R
1 hr – meeting w Ariana about EPIMAR (travel details and timeseries stuff)
3 hr – QSCI381 finish grading assignment 7 (looonng assignment this week)
2 hr – QSCI381 office hours – I included reviewing the Assignment 8 key during this time to check for errors – looks good!
Daily total: 9 hrs
04 March 2025
Tuesday
1.5 hr – SWED102 class
2.5 hr – QSCI381 Lab
6 hr – QSCI381 grading Assignment 7
Daily total: 10 hr
03 March 2025
Monday
1 hr – misc. start-of-week catching up on emails, canvas inbox, ed discussion, slack
1 hr – SWED102 class
2 hr – QSCI381 prep for Lab
2 hr – QSCI381 lab
2 hr – QSCI381 had to create an entirely new Assignment 7 key bc the one Tsering made had errors in like half of the questions
2 hr – QSCI381 grading
Daily total: 10 hr